CMC Correlative Microscopy Coverslips
affordable polyester film cover slips with practical patterns



Introduction

The CMC Correlative Microscopy Coverslips are made using a polyester based film with unique reticle patterns. They are specifically designed to allow indentification and location of particular areas of interest with brightfield or fluorescence microscopes and then processing for electron microscopy.
The material of the CMC correlative microscopy coverslips is compatible with cell culture growth and exhibit good adhesion for cell cultures.

Features and benefits of the CMC correlative microscopy coverslips:

  • good optical quality in brightfield and fluorescence microscopy
  • excellent transparency
  • use temperature range -196°C to +100°C
  • rigid – does not float in the middle of culture
  • resistant to normal chemicals used in electron microscopy
  • no oxygen retention; compatible with LR white resin
  • simple sterilisation using alcohol or UV
  • easy to handle and easy to cut with a knife or punch
  • choice of three different field proven patterns
  • low cost


CMC Correlative Microscopy Coverslips specifications:

Product

CMC 34A

CMC 35

CMC35RND

CMC 71

CMC71RND

CMC1

Product #

51-002834

51-002835

51-002836

51-002871

51-002872

51-002801

Pattern size

5 ea 1x1mm
5x5mm area

10x10mm
100 mm2

10x10mm
100 mm2

10x10mm
100 mm2

10x10mm
100 mm2

10mm

Divisions

0.1x0.1mm

1x1mm

1x1mm

0.5x0.5mm

0.5x0.5mm

0.1mm

Coverslip size

22x22mm

22x22mm

Ø 18mm

22x22mm

Ø 18mm

22x22mm

Thickness

 

0.18mm

 

 

Material

 

Polyester

 

 

The CMC correlative microscopy coverslips are not designed to replace the optically superior quartz or glass coverslips; the film itself has a structure which can be visible in certain illumination conditions. The CMC correlative coverslips are designed for correlative microscopy using different imaging techniques. An initial general analysis is performed using light microscopy and then the sample is further processed for SEM or TEM analysis. The key advantage is that the film is easily cut and punched to allow EM processing.

How to use the CMC correlative microscopy coverslips:

  1. Sterilise the CMC correlative coverslip with alcohol, dry and add culture
  2. Ensure that the grid of the correlative coverslip is positioned correctly and that the text is readable
  3. Record images needed and note the co-ordinates of the region of interest
  4. Fix, dehydrate and embed with resin for TEM
  5. Place a BEEM capsule, filled with reson onto the coverslip over the selected region of interest
  6. Cure resin, detach the correlative coverslip; the footprint of the grid in the resin shows the selected location, trim the block in the selected area and make cuts using an ultramicrotome

Ordering Information for CMC Correlative Microscopy Coverslips

*Prices without VAT, but within the EU, we have to check for valid VAT-ID.
  
CMC01 correlative coverslips 10mm linear scale with 0.1mm divisions
CMC01 correlative coverslips 10mm linear scale with 0.1mm divisions CMC01 - 10mm linear scale with 0.1mm divisions.
Product # Unit Price* Add to Quote / Order
51-002801 CMC01 correlative coverslips 10mm linear scale with 0.1mm divisions
Qty:

pkg/25 €61,00


CMC34A correlative coverslips 5 x 1x1mm with 0.1mm divisions
CMC34A correlative coverslips 5 x 1x1mm with 0.1mm divisions CMC34A - 10 x 10 grids of 0.1mm squares at 5 positions. Indexed 1-10 along the top and A-J down the side. Five positions of 1x1mm each are indexed A-E. Total area 5 x 1mm2
Product # Unit Price* Add to Quote / Order
51-002834 CMC34A correlative coverslips 5 x 1x1mm with 0.1mm divisions
Qty:

pkg/25 €74,00


CMC35 correlative coverslips 10x10mm with 1mm divisions
CMC35 correlative coverslips 10x10mm with 1mm divisions CMC35 - 10 x 10 grid of 1mm squares. Each individually indexed 0-99. Total area 100mm2.
Product # Unit Price* Add to Quote / Order
51-002835 CMC35 correlative coverslips 10x10mm with 1mm divisions
Qty:

pkg/25 €61,00


CMC35RND correlative coverslips 10x10mm with 1mm divisions, Ø 18mm
CMC35RND correlative coverslips 10x10mm with 1mm divisions, Ø 18mm CMC35RND - 10 x 10 grid of 1mm squares. Each individually indexed 0-99. Total area 100mm2. On a Ø 18mm coverslip.
Product # Unit Price* Add to Quote / Order
51-002836 CMC35RND correlative coverslips 10x10mm with 1mm divisions, Ø 18mm
Qty:

pkg/25 €63,00


CMC71 correlative coverslips 10x10mm with 0.5mm divisions
CMC71 correlative coverslips 10x10mm with 0.5mm divisions CMC71 - 20 x 20 grid of 0.5mm squares. Indexed 1-20 along the top, A-T down the side and repeated on centre cross. Total area 100mm2.
Product # Unit Price* Add to Quote / Order
51-002871 CMC71 correlative coverslips 10x10mm with 0.5mm divisions
Qty:

pkg/25 €61,00


CMC71RND correlative coverslips 10x10mm with 0.5mm divisions, Ø 18mm
CMC71RND correlative coverslips 10x10mm with 0.5mm divisions, Ø 18mm CMC71RND - 20 x 20 grid of 0.5mm squares. Indexed 1-20 along the top, A-T down the side and repeated on centre cross. Total area 100mm2. On a Ø 18mm coverslip.
Product # Unit Price* Add to Quote / Order
51-002872 CMC71RND correlative coverslips 10x10mm with 0.5mm divisions, Ø 18mm
Qty:

pkg/25 €63,00




SEM Supplies

TEM Supplies

Calibration

Sample Preparation

AFM / SPM

  Product Menu with Images
  SEM Sample Stubs
  Carbon Tabs
  Sample Stub Adapters
  SEM Stage Adapters
  SEM Sample Holders
  SEM Preparation Stands
  Filaments / Cathodes
  Silicon Finder Grid
  Gatan 3View Pins
  FEI Volumescope Pins
  SEM Stub Storage Boxes
  Phenom Supplies
  JEOL NeoScope Supplies
  Hitachi TM Series Supplies
  Field & Lab Sampler Kits
  FlowView liquid sample Kit

Instruments

  Acoustic Enclosures
  Cressington SEM coaters
  TEM Sample Prep
  Diaphragm Pump
  Rotary vacuum Pump
  Precision diamond saw  
  TEM Grids
  TEM Support Films
  Silicon Nitride Films
  K-Kit Wet Cell Holder
  Graphene Films
  TEM Grid Boxes
  Cryo Grid Boxes
  TEM Sample Staining
  3mm Embedding Tubes
  Filaments/Cathodes
  Eyelash Manipulators

Cryo Supplies

  Cryo Grid Boxes
  Other Cryo Supplies

FIB Supplies

  FIB Lift-out Grids
  FIB Low Profile Stubs
  FIB Grid Holders
  FIB Pre-tilt Holders
  FIB Pre-tilt stubs
  FIB Grid Boxes
  SEM / FIB Magn. Calib.
  SEM Res. Test Spec.
  EDS / WDS Calibration
  TEM Calibration
  AFM / SPM Calibration
  LM Magn. Calibration

Vacuum Supplies

  Vacuum Gauges
  KF/NW Vacuum Parts
  KF/NW Vacuum Hoses
  ISO Vacuum flange parts
  Vacuum Oil and Grease
  Vacuum Sealant
  Diaphragm Pump
  Rotary vacuum Pump
  Vacuum Sample Storage
  Vacuum Pick-Up Pens

Sample Coating Supplies

  Sputter Targets
  Carbon Rods & Fibers
  Quartz QCM Crystals
  SEM Sample Coating Fluid

Evaporation Supplies

  Thermal Evaporation Sources
  Evaporation Materials
  Supports & Substrates
  SEM Preparation Stands
  Tweezers
  Probes & Picks
  Applicators & Swabs
  Plastic Transfer Pipettes
  Cutting Tools / Scissors
  Conductive Paint/Cement
  Conductive Tapes & Tabs
  Non-Conductive Adhesives
  Sample Storage
  Hand Tools
  Sorting Wire Mesh
  Preparation Surfaces
  Cleaning / Gloves
  Conductive Metal Powders
  Metallographic Sample Prep
  AFM/SPM Discs
  AFM/SPM Disc Pick-up Tool
  AFM/SPM Holders
  AFM/SPM Disc Storage
  Cantilever Tweezers
  AFM/SPM Discs Tweezers
  Nano-Tec Mica Discs
  HOPG Substrates

Light Microscopy

  Correlative Cover Slips
  Glass Microscope Slides
  Glass Cover Slips
  Quartz Microscope Slides
  Quartz Cover Slips
  Black Metal Slides
  Slide Storage Boxes
  LM Calibration
  Plastic Transfer Pipettes
  Optical Lens Tissue
  Glass Petri Dishes
  Digital Microscope
  PTFE Beakers