Conductive SEM Coating Fluid
Cost-effective alternative to vacuum sputter coating

  Conductive SEM Coating Fluid     

The BEL-1 conductive ionic SEM coating fluid offers a simple and cost-effective alternative for coating non-conductive sample with a conductive surface layer. Instead of using a sputter coater with a precious metal target, the sample needs to be immersed in a diluted conductive SEM coating solution for several minutes.

Features of the BEL-1 conductive ionic SEM coating fluid are:

  • Excellent permeability and high electrical conductivity
  • Water-soluble and environmentally friendly coating agent for SEM observation
  • Compatible with most non-conductive materials and fixed biological samples
  • Based on polythiophene conductive polymer
  • Conductivity: 250 S/cm
  • Efficient in use
  • Dilution, immersion and drying procedures can enhance conductivity
  • Best results obtained with 1-3kV SEM beam voltage


The BEL-1 conductive ionic SEM coating fluid needs a 10-20x dilution when used. Only prepare the amount which is directly needed and do not store diluted fluids. The sample must be immersed and completely dried afterwards using a blower.
Storage of the BEL-1 conductive ionic SEM coating fluid should be preferably at 4-8 °C, but since it is water based: Do not Freeze.

Rat Kidney stained Glomerulus
 Rat Kidney stained Glomerulus

Sample preparation conditions (4 ° C): After fixing with 2% glutaraldehyde, shred (thickness approx. 0.5 mm) and re-fix ⇒ Fix with 1% osmium tetroxide 2 hours ⇒ DW wash 10 minutes, 6 times ⇒ 1% tannic acid Aqueous solution 3 hours ⇒ DW washing 10 minutes, 6 times ⇒ 1% osmium tetroxide fixation 2 hours ⇒ DW washing 10 minutes, 3 times ⇒ 2% BEL-1 (70% ethanol) 2 hours immersion ⇒ Blower, cold air dryer drying Observation conditions : SE detector (mixed mix), acceleration voltage 3KV, WD20, emission current (0.8 to 4.7μA)

Kimwipe stained

BEL-1 7% (diluted solution: DW) soaked for 5 minutes. Drying: blower, cold air dryer

More examples of biological sample conductive coating More examples of material sample conductive coating

Courtesy of St. Marianna University School of Medicine, Electron Microscope Research Facility Chisako Sasaki

Technical Support Bulletin: BEL-1 Conductive SEM Coating Fluid

Ordering information for BEL-1 conductive ionic SEM coating fluid

*Prices without VAT, but within the EU, we have to check for valid VAT-ID.
BEL-1 Conductive SEM coating fluid, 10ml	 bottle BEL-1 Conductive SEM coating fluid, 10ml bottle
Product # Unit Price* Add to Quote / Order
52-008001 BEL-1 Conductive SEM coating fluid, 10ml bottle

each €259,00

Hazardous Symbol Explanation

hazard fee symbol = Hazard Shipping Fee
Cg = Suspected Carcinogen
F = Flammable
I = Irritant
S = Sensitizer
T = Toxic
Extra info 52-008001 :   SDS pdf download


SEM Supplies

TEM Supplies


Sample Preparation


  Product Menu with Images
  SEM Sample Stubs
  Carbon Tabs
  Sample Stub Adapters
  SEM Stage Adapters
  SEM Sample Holders
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  Silicon Finder Grid
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  TEM Sample Prep
  Diaphragm Pump
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  K-Kit Wet Cell Holder
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  TEM Sample Staining
  3mm Embedding Tubes
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Cryo Supplies

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FIB Supplies

  FIB Lift-out Grids
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  FIB Grid Holders
  FIB Pre-tilt Holders
  FIB Pre-tilt stubs
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  SEM / FIB Magn. Calib.
  SEM Res. Test Spec.
  EDS / WDS Calibration
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  AFM / SPM Calibration
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Vacuum Supplies

  Vacuum Gauges
  KF/NW Vacuum Parts
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  Diaphragm Pump
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Sample Coating Supplies

  Sputter Targets
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  SEM Sample Coating Fluid

Evaporation Supplies

  Thermal Evaporation Sources
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  Supports & Substrates
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  Probes & Picks
  Applicators & Swabs
  Plastic Transfer Pipettes
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  AFM/SPM Discs
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Light Microscopy

  Correlative Cover Slips
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  Slide Storage Boxes
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