Conductive SEM Coating Fluid
Cost-effective alternative to vacuum sputter coating


  Conductive SEM Coating Fluid     
Introduction

The BEL-1 conductive ionic SEM coating fluid offers a simple and cost-effective alternative for coating non-conductive sample with a conductive surface layer. Instead of using a sputter coater with a precious metal target, the sample needs to be immersed in a diluted conductive SEM coating solution for several minutes.

Features of the BEL-1 conductive ionic SEM coating fluid are:

  • Excellent permeability and high electrical conductivity
  • Water-soluble and environmentally friendly coating agent for SEM observation
  • Compatible with most non-conductive materials and fixed biological samples
  • Based on polythiophene conductive polymer
  • Conductivity: 250 S/cm
  • Efficient in use
  • Dilution, immersion and drying procedures can enhance conductivity
  • Best results obtained with 1-3kV SEM beam voltage

 

The BEL-1 conductive ionic SEM coating fluid needs a 10-20x dilution when used. Only prepare the amount which is directly needed and do not store diluted fluids. The sample must be immersed and completely dried afterwards using a blower.
Storage of the BEL-1 conductive ionic SEM coating fluid should be preferably at 4-8 °C, but since it is water based: Do not Freeze.

Rat Kidney stained Glomerulus
 Rat Kidney stained Glomerulus

Sample preparation conditions (4 ° C): After fixing with 2% glutaraldehyde, shred (thickness approx. 0.5 mm) and re-fix ⇒ Fix with 1% osmium tetroxide 2 hours ⇒ DW wash 10 minutes, 6 times ⇒ 1% tannic acid Aqueous solution 3 hours ⇒ DW washing 10 minutes, 6 times ⇒ 1% osmium tetroxide fixation 2 hours ⇒ DW washing 10 minutes, 3 times ⇒ 2% BEL-1 (70% ethanol) 2 hours immersion ⇒ Blower, cold air dryer drying Observation conditions : SE detector (mixed mix), acceleration voltage 3KV, WD20, emission current (0.8 to 4.7μA)

Kimwipe stained
Kimwipe

BEL-1 7% (diluted solution: DW) soaked for 5 minutes. Drying: blower, cold air dryer

More examples of biological sample conductive coating More examples of material sample conductive coating

Courtesy of St. Marianna University School of Medicine, Electron Microscope Research Facility Chisako Sasaki



Ordering information for BEL-1 conductive ionic SEM coating fluid

*Prices without VAT, but within the EU, we have to check for valid VAT-ID.
  
BEL-1 Conductive SEM coating fluid, 10ml	 bottle BEL-1 Conductive SEM coating fluid, 10ml bottle
Product # Unit Price* Add to Quote / Order
52-008001 BEL-1 Conductive SEM coating fluid, 10ml bottle
Qty:

each €259,00

Hazardous Symbol Explantion

hazard fee symbol = Hazard Shipping Fee
Cg = Suspected Carcinogen
F = Flammable
I = Irritant
S = Sensitizer
T = Toxic
Extra info 52-008001 :   SDS pdf download

 



SEM Supplies

TEM Supplies

Calibration

Sample Preparation

AFM / SPM

  Product Menu with Images
  SEM Sample Stubs
  Carbon Tabs
  Sample Stub Adapters
  SEM Stage Adapters
  SEM Sample Holders
  SEM Preparation Stands
  Filaments / Cathodes
  Silicon Finder Grid
  Gatan 3View Pins
  FEI Volumescope Pins
  SEM Stub Storage Boxes
  Phenom Supplies
  JEOL NeoScope Supplies
  Hitachi TM Series Supplies
  Field & Lab Sampler Kits
  FlowView liquid sample Kit

Instruments

  Acoustic Enclosures
  Cressington SEM Coaters
  TEM Sample Prep
  TEM Grids
  TEM Support Films
  Silicon Nitride Films
  K-Kit Wet Cell Holder
  Graphene Films
  TEM Grid Boxes
  Cryo Grid Boxes
  TEM Sample Staining
  3mm Embedding Tubes
  Filaments/Cathodes
  Eyelash Manipulators

Cryo Supplies

  Cryo Grid Boxes
  Other Cryo Supplies

FIB Supplies

  FIB Lift-out Grids
  FIB Low Profile Stubs
  FIB Grid Holders
  FIB Pre-tilt Holders
  FIB Grid Boxes
  SEM / FIB Magn. Calib.
  SEM Res. Test Spec.
  EDS / WDS Calibration
  TEM Calibration
  AFM / SPM Calibration
  LM Magn. Calibration

Vacuum Supplies

  Bullseye Vacuum Gauges
  Quick-check Gauges
  KF/NW Vacuum Parts
  KF/NW Vacuum Hoses
  Vacuum Oil and Grease
  Vacuum Sealant
  Vacuum Sample Storage

Sample Coating Supplies

  Sputter Targets
  Carbon Rods & Fibers
  Quartz QCM Crystals
  SEM Sample Coating Fluid

Evaporation Supplies

  Thermal Evaporation Sources
  Evaporation Materials
  Supports & Substrates
  SEM Preparation Stands
  Tweezers
  Probes & Picks
  Applicators & Swabs
  Plastic Transfer Pipettes
  Cutting Tools / Scissors
  Conductive Paint/Cement
  Conductive Tapes & Tabs
  Non-Conductive Adhesives
  Sample Storage
  Hand Tools
  St. St. Woven Wire Mesh
  Preparation Surfaces
  Cleaning / Gloves
  Replication Sheets
  Conductive Metal Powders
  Metallographic Sample Prep
  AFM/SPM Discs
  AFM/SPM Disc Pick-up Tool
  AFM/SPM Holders
  AFM/SPM Disc Storage
  Cantilever Tweezers
  AFM/SPM Discs Tweezers
  Nano-Tec Mica Discs
  HOPG Substrates

Light Microscopy

  Correlative Cover Slips
  Glass Microscope Slides
  Glass Cover Slips
  Quartz Microscope Slides
  Quartz Cover Slips
  Black Metal Slides
  Slide Storage Boxes
  LM Calibration
  Plastic Transfer Pipettes
  Optical Lens Tissue
  Glass Petri Dishes